WebEvidence suggests that Succinylated Wheat Germ agglutinin does not bind to sialic acid residues, unlike the native form, but retains its specificity toward N-acetylglucosamine. Fluorescein labeled, succinylated WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. WebJan 1, 1980 · Results indicated that WGA bound to these cells in a complex fashion described by a curvilinear (upwardly concave) Scatchard plot and a nonlinear dissociation curve. Binding was rapid and reversible and was inhibited 80–90% by 0.1 MN-acetylglucosamine, ... FITC. fluorescein isothiocyanate. FMF. flow microfluorometry. …
Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat ...
WebMay 16, 2024 · Slides are then incubated for 2 h at room temperature with FITC-WGA solution. 4. After removing the WGA solution, samples are washed three times for 10 min in PBS. 5. PBS is replaced with a few drops of PI solution (approximately 50 μL) and an equivalent volume of antifade reagent. WebJan 10, 2024 · FITC (was one of the first dyes which was used for fluorescence microscopy and served as a precursor for other fluorescent dyes like Alexa Fluor®488. Its fluorescence activity is due to its large conjugated aromatic electron system, which is excited by light in the blue spectrum. ... (WGA) which binds specifically to sialic acid and N ... ray krebbs house on dallas
Fluorescein (FITC) Thermo Fisher Scientific - US
http://www.rx-8.com/chanpinzhongxin/210133.html WebNov 19, 2015 · Among seven different lectins we examined, FITC labeled Triticum vulgaris (wheat germ) agglutinin (WGA) delineated the GCX most clearly. Binding of WGA to the GCX was inhibited by chitin hydrolysate, which … WebAug 26, 2024 · Cardiomyocytes in the cardiac sections were outlined by FITC-conjugated wheat germ agglutinin (WGA) that binds to glycoproteins of the cell membrane, and is routinely used for the staining of cardiac sarcolemma to determine cross sectional area or myocyte density . Staining was visualised under the A1R confocal microscope (Nikon, … ray kroc family