Fluoromount sigma
WebJan 30, 2024 · The sections were rinsed with 0.3% eriochrome-black T in 1× PBS to completely block natural collagen fluorescence and they were then mounted with cover-glass with Fluoromount (Sigma-Aldrich, Saint-Louis, MO, USA). Negative controls were performed by omitting primary antibodies in the same conditions that were previously … WebDAPI Fluoromount-G ® is a water-soluble, 4',6-diamidino-2-phenylindole (DAPI) containing compound for slides mounted after a staining procedure having an aqueous final step. It is suitable for microscopy applications utilizing frozen and paraffin sections, whole mounts, and cells requiring nuclear visualization. Add to Cart
Fluoromount sigma
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WebFeb 25, 2024 · Slides were then fixed for 5 min in 10% formalin, blocked for 30 min in 0.2% Triton, 2% cold water fish gelatin (Sigma, St. Louis, MO, USA ... Invitrogen, Carlsbad, CA, USA). Slides were mounted with DAPI Fluoromount-G (SouthernBiotech, Birmingham, AL, USA). Representative confocal images were taken on a Nikon C2 at 20× magnification … WebMar 10, 2024 · Samples were mounted with Fluoromount (Sigma-Aldrich, San Louis, MO, USA, Cat#F4680). Images were acquired with 63× objective 1.35 N.A. using optical sectioning and structured illumination Zeiss Apotome 2, Axiovert 7.
WebApr 14, 2024 · Necrostatin-1 (Sigma, N9037) was dissolved in DMSO and cells were treated at a final concentration of 20 µM. GSK2656157 (SelleckChem, S7033) was dissolved in DMSO and cells were treated at a ... WebSigma-Aldrich - S7653 Page 1 of 8 The life science business of Merck KGaA, Darmstadt, Germany operates as MilliporeSigma in the US and Canada SAFETY DATA SHEET Version 6.10 Revision Date 02/21/2024 Print Date 04/08/2024 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifiers
WebApr 18, 2024 · After that, samples were thoroughly rinsed in PBS and incubated with 1:500 Alexa Fluor 488 donkey-anti mouse IgG (Invitrogen, ThermoFisher Scientific, US) for 2 h at room temperature. Then samples were rinsed in PBS and coverslipped with mounting medium (FluoroMount, Sigma, USA). Omission of primary antibodies was used as IHC … WebA special mounting media for immunofluorescent staining procedures. It is a water soluble, non-fluorescing medium which is well suited for use when the staining procedure has an aqueous final step. It contains 10% polyvinyl alcohol in phosphate buffered glycerol with 0.1% sodium azide as a… Related Products: Mounting Medium Compare this item
WebFluoromount ™ Aqueous Mounting Medium has been used for mounting: coverslips on slides for immunofluorescence microscopic examination of PC12 and Hela cells [ 1] hippocampus neurons cells for confocal microscope studies [ 2] brain sections for immunofluorescence staining [ 3] Envase 25 mL Información legal
WebFluoromount-G ® is a water-soluble compound for slides mounted after a staining procedure having an aqueous final step. It is suitable for microscopy applications utilizing … florida health science centerWebDec 23, 2015 · Finally, cells were stained with DAPI nucleic acid stain (4′,6-diamidino-2-phenylindole, Invitrogen, Carlsbad, CA, USA) and mounted using Fluoromount mounting medium (Sigma). Samples were analyzed using the Leica TCS SP5 confocal laser-scanning microscope (Leica, Wetzlar, Germany), and the resulting images were processed using … great wall of californiaWebApr 7, 2024 · Sigma antibodies used were ULK1 (#A7481), β-actin (#A5541), Mitofusin 2 (#M6319), and Flag tag polyclonal (#F7425). MitoNEET/CISD1 (#16006-1-AP) and total MFF (#17090-1-AP) antibodies were from ProteinTech. ... #D9542). Coverslips were mounted with Fluoromount-G (Thermo Fisher Scientific, #OB10001). Images were … florida health seminole countyWebProperties form viscous liquid technique (s) immunocytochemistry: suitable Description Application For use in Mycoplasma stain kit. Components Contains sodium phosphate and citric acid in glycerol. Safety Information Storage Class Code 10 - Combustible liquids WGK nwg Flash Point (F) Not applicable Flash Point (C) Not applicable Documentation florida health plans insuranceWebOct 4, 2024 · Giemsa stain is a differential stain and contains a mixture of azure, methylene blue, and eosin dye. It is specific for the phosphate groups of DNA and attaches itself to where there are high amounts of adenine-thymine bonding. Azure and eosin are acidic dye that variably stains the basic components of the cells like the cytoplasm, granules, etc. florida health solution cancellationWebNuclei were labeled with DAPI Fluoromount (Sigma Aldrich, Burlington, MA, USA). 4.2.4. Western Blot Analysis. Three weeks after TBI induction, brain extracts were homogenized and dissociated in a buffer lysis (Tissue Protein extraction Reagent, Pierce San Antonio, TX, USA). Using a Teflon pestle homogenizer, these actions were supplemented with ... florida health sciences center tampa flWebFluoromount ™ Aqueous Mounting Medium has been used for mounting: coverslips on slides for immunofluorescence microscopic examination of PC12 and Hela cells; … great wall of china 1960