WebDNA interstrand crosslinks (ICLs) covalently link the two strands of a DNA duplex, thereby acting as an absolute block to DNA helicases and polymerases. As a result, ... WebMay 30, 2024 · The replication-associated AP site repair protein HMCES protects cells from strand breaks, inhibits mutagenic translesion synthesis, and participates in repair of …

Interstrand Cross-Links Generated by Abasic Sites in Duplex DNA ...

WebInterstrand DNA crosslinks (ICLs) are a toxic form of DNA damage that block DNA replication and transcription by tethering the opposing … WebThe effect of the combined antimetabolites on the level and persistence of DDP-induced DNA interstrand cross-links was assessed by DNA alkaline elution. These were measured as an indicator of DDP-DNA adduct formation and removal. When HU + ara-C exposure preceded or followed DDP treatment, ... susan castle obituary

FANCM interacts with PCNA to promote replication traverse of DNA ...

WebMay 8, 2009 · In contrast, the cytotoxic-inactive complex 2 preferentially forms 1,4-intrastrand rather than 1,3-intra- and -interstrand cross-links. Digestion of the DNA adducts of 1 shows that the cleavage is completely blocked at one nucleotide before the cross-linked guanine residues on the opposite strand, ... WebJan 7, 2005 · DNA interstrand crosslinks are cytotoxic adducts formed naturally and by drugs used in antitumor therapy. Our current knowledge of the cellular responses … WebMitomycin C, (MC), an antitumor drug used in the clinics, is a DNA alkylating agent. Inert in its native form, MC is reduced to reactive mitosenes in cellulo which undergo nucleophilic attack by DNA bases to form monoadducts as well as interstrand crosslinks (ICLs). These properties constitute the m … susan castleberry