WebFor a 6-well plate, we incubated 2 µg of plasmid DNA with 125 µL of Opti-MEM (Thermo Fisher Scientific) at room temperature for 5 min (min). We incubated this mixture with 10 µL of Lipofectamine 2000 in 115 µL of Opti-MEM at room temperature for 45 min. We added all 250 µL of the DNA–lipid mixture to a well of a 6-well plate and then ... WebOpti-MEM I Reduced Serum Medium Lipofectamine® RNAiMAX Transfection Reagent Thermo Fisher Scientific (13778150) siRNA (10 µm/L) of interest serum-free cell culture media . Forward Transfection Use this procedure to forward transfect siRNA into mammalian cells in a 24-well format (for other formats, see Scaling Up or Down …
Lipofectamine® LTX & PLUS™ Reagent - Thermo Fisher …
WebDec 27, 2014 · Because we have 4 siRNAs, we will prepare a master mix of RNAiMAX. Add 200 microliters of Opti-MEM medium and 12 micoliters of RNAiMAX in a tube labelled "Master Mix" Mix well by vortexing or flicking the tube. Add 50 microliters of Opti-MEM Medium into each of 4 tubes and label them 1, 2, Positive, and Negative. WebOpti-MEM I medium 25 µL Lipofectamine 3000 reagent 2 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0.5–5 µg/µL) 250 ng P3000™ reagent 0.5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; how do i get a covid certificate in wales
Opti-MEM™ I Reduced Serum Medium from Thermo Fisher Scientific
WebMar 31, 2024 · CSII-EF-IN was converted to a Gateway destination vector by the insertion of the RfA cassette (11828029, Thermo Fisher Scientific). MYADM cDNAs were transferred from pENTR to CSII-EF-IN using LR Clonase (11791020, Thermo Fisher Scientific). pBluescript II SK (+) was used as the cloning vector (212205, Agilent). WebMar 10, 2024 · BY-2-xGxFL cells were collected in a tube and washed once with Opti-MEM I (Thermo Fisher Scientific, Waltham, MA, USA). Cells with 20 µL of PCV were suspended in 200 µL of Opti-MEM I with HNCre protein on ice. HNCre protein concentrations were 5 µM to evaluate the poring pulse voltage and length effect, were 0.1, 0.2, 0.5, 1, 2, or 5 µM to ... WebOpti-MEM I减血清培养基. Gibco™ Opti-MEM™ I减血清培养基是Eagle最小必需培养基的改良版,以HEPES和碳酸氢钠为缓冲液,并添加了次黄嘌呤、胸苷、丙酮酸钠、L-谷氨酰胺、 … how much is the autocad software